Formate tuaj yeem pom tau tias yog tus txha nraub qaum ntawm kev lag luam bioeconomy uas tsis muaj carbon, tsim los ntawm CO2 siv cov txheej txheem (electro) tshuaj lom neeg thiab hloov mus rau cov khoom muaj nqis ntxiv siv cov enzymatic cascades lossis cov kab mob me me. Ib kauj ruam tseem ceeb hauv kev nthuav dav kev sib xyaw ntawm cov qauv tsim yog nws qhov kev txo qis thermodynamically ntawm formaldehyde, uas ntawm no tshwm sim ua qhov hloov xim daj. Credit: Lub Koom Haum ntawm Terrestrial Microbiology Max Planck / Geisel.
Cov kws tshawb fawb ntawm Max Planck Institute tau tsim ib txoj hauv kev ua kom cov pa roj carbon dioxide hloov mus ua formaldehyde nrog kev pab los ntawm formic acid, muab txoj hauv kev tsis muaj carbon los tsim cov khoom muaj nqis.
Cov txheej txheem tshiab rau kev kho cov pa roj carbon dioxide tsis yog tsuas yog pab txo cov pa roj carbon dioxide hauv huab cua xwb, tab sis kuj tseem tuaj yeem hloov cov tshuaj ib txwm muaj ntawm cov tshuaj thiab cov khoom xyaw nquag nrog cov txheej txheem biological uas tsis muaj carbon. Kev tshawb fawb tshiab qhia txog cov txheej txheem uas formic acid tuaj yeem siv los hloov cov pa roj carbon dioxide mus rau hauv cov khoom siv muaj nqis rau kev lag luam biochemical.
Vim muaj kev nce ntxiv ntawm cov pa roj av uas ua rau lub ntiaj teb sov, kev rho cov pa roj carbon lossis kev rho cov pa roj carbon dioxide los ntawm cov chaw tso pa tawm loj yog qhov teeb meem tseem ceeb. Hauv xwm, kev sib xyaw cov pa roj carbon dioxide tau ua mus txog ntau lab xyoo, tab sis nws lub zog tseem tsis txaus los them rau cov pa roj uas tib neeg ua.
Cov kws tshawb fawb uas coj los ntawm Tobias Erb ntawm Lub Koom Haum ntawm Terrestrial Microbiology. Max Planck siv cov cuab yeej ntuj tsim los tsim cov txheej txheem tshiab rau kev kho cov pa roj carbon dioxide. Tam sim no lawv tau ua tiav hauv kev tsim ib txoj hauv kev ua kom lub cev zom zaub mov uas tsim cov formaldehyde uas muaj zog heev los ntawm formic acid, uas yog ib qho nruab nrab hauv kev ua kom lub cev zom zaub mov. Formaldehyde tuaj yeem nkag mus rau hauv ntau txoj hauv kev ua kom lub cev zom zaub mov los tsim lwm yam khoom muaj nqis yam tsis muaj kev cuam tshuam lom. Ib yam li cov txheej txheem ntuj tsim, ob yam khoom xyaw tseem ceeb yog xav tau: lub zog thiab carbon. Thawj qhov tuaj yeem muab tsis yog los ntawm lub hnub ci ncaj qha xwb, tab sis kuj los ntawm hluav taws xob - piv txwv li, cov khoom siv hnub ci.
Hauv cov saw hlau muaj nqis, cov pa roj carbon muaj ntau yam sib txawv. Cov pa roj carbon dioxide tsis yog tib txoj kev xaiv ntawm no, peb tab tom tham txog txhua yam pa roj carbon sib xyaw (C1 building blocks): carbon monoxide, formic acid, formaldehyde, methanol thiab methane. Txawm li cas los xij, yuav luag txhua yam ntawm cov tshuaj no muaj tshuaj lom heev, rau cov kab mob muaj sia (carbon monoxide, formaldehyde, methanol) thiab rau lub ntiaj teb (methane ua cov pa roj av). Tsuas yog tom qab formic acid tau raug neutralized rau nws cov qauv yooj yim uas ntau cov kab mob me me zam tau cov concentration siab ntawm nws.
"Formic acid yog ib qho chaw muaj carbon zoo heev," Maren Nattermann, tus sau thawj zaug ntawm txoj kev tshawb fawb no hais tias. "Tab sis kev hloov nws mus rau formaldehyde hauv vitro yog siv zog heev." Qhov no yog vim formate, ntsev ntawm formate, tsis yooj yim hloov mus rau formaldehyde. "Muaj ib qho teeb meem tshuaj lom neeg loj heev ntawm ob lub molecule no, thiab ua ntej peb tuaj yeem ua qhov kev tshuaj tiv thaiv tiag tiag, peb yuav tsum kov yeej nws nrog kev pab los ntawm lub zog biochemical - ATP."
Lub hom phiaj ntawm cov kws tshawb fawb yog nrhiav txoj hauv kev pheej yig dua. Tom qab tag nrho, lub zog tsawg dua uas xav tau los pub cov pa roj carbon rau hauv kev ua haujlwm, lub zog ntau dua tuaj yeem siv los txhawb kev loj hlob lossis kev tsim khoom. Tab sis tsis muaj txoj hauv kev zoo li no hauv xwm. "Kev tshawb pom ntawm cov enzymes sib xyaw nrog ntau lub luag haujlwm xav tau qee qhov kev muaj tswv yim," Tobias Erb hais. "Txawm li cas los xij, kev tshawb pom cov enzymes sib tw tsuas yog qhov pib xwb. Peb tab tom tham txog cov tshuaj tiv thaiv uas tuaj yeem suav ua ke vim tias lawv qeeb heev - qee zaum, muaj tsawg dua ib qho tshuaj tiv thaiv ib ob rau ib enzyme. Cov tshuaj tiv thaiv ntuj tuaj yeem ua mus ntxiv ntawm tus nqi uas yog ib txhiab npaug sai dua. "Qhov no yog qhov uas cov tshuaj biochemistry tsim los, Maren Nattermann hais tias: "Yog tias koj paub cov qauv thiab cov txheej txheem ntawm ib qho enzyme, koj paub qhov twg yuav cuam tshuam. Nws tau muaj txiaj ntsig zoo heev."
Kev ua kom zoo dua ntawm cov enzyme muaj ntau txoj hauv kev: kev sib pauv tshwj xeeb ntawm cov khoom siv hauv tsev, kev hloov pauv tsis raws cai, thiab kev xaiv peev xwm. "Ob qho tib si formate thiab formaldehyde yog qhov tsim nyog heev vim tias lawv tuaj yeem nkag mus rau hauv cov phab ntsa cell. Peb tuaj yeem ntxiv formate rau hauv cov kab lis kev cai cell, uas tsim cov enzyme uas hloov cov formaldehyde ua rau cov xim daj tsis muaj tshuaj lom tom qab ob peb teev, "Maren hais. Nattermann piav qhia.
Cov txiaj ntsig hauv lub sijhawm luv luv no yuav tsis ua tau yog tsis siv cov txheej txheem siab. Txhawm rau ua qhov no, cov kws tshawb fawb tau koom tes nrog tus khub lag luam Festo hauv Esslingen, Lub Tebchaws Yelemees. "Tom qab kwv yees li 4,000 qhov kev hloov pauv, peb tau nce peb cov txiaj ntsig plaub npaug," Maren Nattermann hais. "Yog li, peb tau tsim lub hauv paus rau kev loj hlob ntawm tus qauv kab mob E. coli, tus kab mob ua haujlwm ntawm biotechnology, ntawm formic acid. Txawm li cas los xij, tam sim no, peb cov hlwb tsuas tuaj yeem tsim formaldehyde thiab tsis tuaj yeem hloov pauv ntxiv."
Ua ke nrog nws tus neeg koom tes Sebastian Wink los ntawm Lub Tsev Kawm Ntawv ntawm Cov Nroj Tsuag Molecular Physiology. Cov kws tshawb fawb Max Planck tam sim no tab tom tsim ib hom kab mob uas tuaj yeem siv cov khoom nruab nrab thiab qhia lawv rau hauv lub cev metabolism. Tib lub sijhawm, pab pawg neeg tab tom ua kev tshawb fawb txog kev hloov pauv electrochemical ntawm carbon dioxide mus rau formic acid nrog ib pawg neeg ua haujlwm ntawm Lub Tsev Kawm Ntawv ntawm Kev Hloov Pauv Zog Tshuaj. Max Planck nyob rau hauv kev coj ntawm Walter Leitner. Lub hom phiaj ntev yog "ib qho loj-haum-txhua lub platform" los ntawm carbon dioxide tsim los ntawm cov txheej txheem electrobiochemical rau cov khoom xws li insulin lossis biodiesel.
Siv: Maren Nattermann, Sebastian Wenk, Pascal Pfister, Hai He, Seung Hwang Lee, Witold Szymanski, Nils Guntermann, Faiying Zhu “Kev tsim kho ntawm ib qho cascade tshiab rau kev hloov pauv ntawm phosphate-dependent formate mus rau formaldehyde hauv vitro thiab hauv vivo”, Lennart Nickel. , Charlotte Wallner, Jan Zarzycki, Nicole Pachia, Nina Gaisert, Giancarlo Francio, Walter Leitner, Ramon Gonzalez, thiab Tobias J. Erb, Tsib Hlis 9, 2023, Nature Communications.DOI: 10.1038/s41467-023-38072-w
SciTechDaily: Lub Tsev ntawm cov xov xwm thev naus laus zis zoo tshaj plaws txij li xyoo 1998. Nyob twj ywm hloov kho cov xov xwm thev naus laus zis tshiab tshaj plaws los ntawm email lossis social media. > Email digest nrog kev sau npe dawb
Cov kws tshawb fawb ntawm Cold Spring Harbor Laboratories pom tias SRSF1, ib qho protein uas tswj hwm RNA splicing, raug tswj hwm hauv lub txiav.